15+ Oligo Resuspension Calculator
Total nmol µg of oligo x 1 µmolMW x 1000 nmol1 µmol Note. Quickly calculate your oligos molecular weight and extinction coefficient.
Relationship Between Average Coupling Efficiency And Synthesis Yield Y Download Scientific Diagram
Use the IDT Resuspension Calculator under the Tools tab of the.
. Select DNA or RNA paste your sequence and click calculate for the OligoEvaluator tool to return values. Web Oligo Resuspension Calculator Determine volumes for resuspending lyophilized oligos to a desired concentration Tm Prediction Predict the melting temperature for LNA oligos. Web number of nanomoles using the amount of oligo in µg and the molecular weight as follows.
Web Oligo Resuspension Calculator. Web An oligo resuspension calculator is a tool used to determine the volume of the diluent needed to dissolve a given amount of oligonucleotides short DNA or RNA. Web OligoEvaluator is easy to use.
Web The OligoSpec calculator provides the physical properties for your oligo design. Calculate T m identify secondary structure optimize codon use. Web Revisedupdated Jul 27 2022.
Web IDT offers a Resuspension Calculator as part of our SciTools suite which is useful for calculating the volume of buffer to add to a dried down oligo to obtain a. Easily create a stock solution by allowing the. Web Resuspension calculator Figure 2.
Enter the quantity that you calculated. This tool can be used to determine how much water or buffer is needed to resuspend a dry lyophilized oligo to obtain your desired final. Web The oligo can be dissolved in TE buffer 10 mM Tris pH 80 01 mM EDTA or molecular biology grade water.
Select Sgl-Stranded DNA single. Web The OligoCalc DNA MW calculations assume that there is not a 5 monophosphate Anhydrous Molecular Weight A n x 31321 T n x 3042 C n x. Quickly calculate your oligos molecular weight and extinction coefficient.
1 mole 103 mmoles. Web Oligos should be resuspended in TE Buffer 10mM TrisHCl 1 mM EDTA pH 80 Recommended or DNase-free water. Web Free tools for oligo design handling and storage to help you get the most out of your oligos.
Web The OligoSpec calculator provides the physical properties for your oligo design. Determines how much volume uL to resuspend a dry lyophilized oligo in. Web Easily create an stock solution by allowing the resuspension computing take the guesswork exit of dissolving your oligo.
Enter your final concentration per oligo that you calculated. Get these tips from IDTs scientists on the best ways to resuspend quantify and calculate copy number of IDT Gene Fragments. All reported properties are.
Web The Resuspension Calculator assists in determining the volume needed to resuspend a dry lyophilized oligo to a desired concentration.
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